Sakuma and Yamamoto tell "magic wands of CRISPR in CBT

2017-08-27

Sakuma and Yamamoto as respected experts demonstrate that genome editing-assisted gene knock-in technology has become rather diversified and improved from day to day in Cell Biol Toxicol (https://link.springer.com/article/10.1007/s10565-017-9409-6). Since genome editing relies on the endogenous DSB repair machineries, their appropriate utilization is a key to control the editing outcome. The favorable DSB repair pathway for gene knock-in is variable depending on various parameters such as the target cell types and organisms, purpose of gene knock-in, and delivery methods. The adequate system should be chosen by the researchers themselves with a deep and correct knowledge to make the most of genome editing and gene knock-in technologies.